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The purpose of this study was to investigate the effect of nardosinone (Nar) on the proliferation, migration and differ entiation of mouse neural stem cells (NSC).The NSC were iso lated from mice embryos at gestational day El4 and cultured u sing the neurosphere culture system.Nar or vehicle was added to the culture media at the start of culture.Cell viability and prolif eration were measured with MTT and BrdU incorporation assays respectively.Cell cycle was analyzed by flow cytometric cell sor ting.The NSC migration and differentiation were monitored with immune-fluorescence techniques.ERK/CREB signaling pathway was detected with Western blotting analysis.The results demon strate that after 3 d incubation with the cells, Nar 50 μκmol· L-1increased the viability, BrdU incorporation, and the proportion of cells in S and G2 phase (P < 0.05).Exposure to Nar for 24 h promoted cell migration in a dose-dependent manner.