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Aim:To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis (EAE),and to study the role NKT cells play in the immunoregulation of EAE.Significance:Our observation on the amount of NKT cells in EAE mice compared with that in normal ones will solid the experimental foundation of NKT cells in EAE and further the study of NKT cells immunoregulation in EAE and other autoimmune diseases.