穿膜肽功能化的长余辉纳米粒子标记J774A.1巨噬细胞归航炎症组织的研究

来源 :第十一届全国化学生物学学术会议 | 被引量 : 0次 | 上传用户:ctbt010
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  生命体内的炎症反应可以招募白细胞,但这一生理过程仍然存在许多不明确的、有歧义的地方,对白细胞进行有效的标记对于了解白细胞募集到炎症组织的过程至关重要.我们设计了羧基化硅烷和TAT 细胞穿膜肽功能化的近红外光发射的长余辉纳米探针(PLNP-TAT),用于标记J774A.1 巨噬细胞并追踪其归航到炎症组织.功能化的近红外长余辉纳米探针免原位激发,并可用红光LED 灯重复激励,成像前激发2 min,成像时无组织自发荧光干扰,并且避免了可能由激发光带来的组织损伤.
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