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目的:探讨高迁移率族蛋白B1(high morbility group protein box 1,HMGB1)刺激对人气道平滑肌细胞(human airway smooth muscle cells,HASMC)增殖和迁移的影响及其机制。方法:体外培养HASMC,将纯化的第3~8代细胞随机分为对照组、HMGB1组(125、250、500和1 000 ng/m L)、TGF-β阳性对照组(1 ng/m L),CCK-8法测定HASMC增殖能力,Transwell小室观察HMGB1对HASMC迁移的影响,Western blot检测HASMC的Akt磷酸化。结果 :与对照组相比,HMGB1呈浓度依赖性刺激HASMC增殖(P<0.05);HMGB1(500 ng/m L)刺激HASMC后,细胞迁移能力显著高于对照组(P<0.01);HMGB1(500 ng/m L)组HASMC的Akt磷酸化水平较对照组显著增高(P<0.05)。结论 :HMGB1促进HASMC增殖和迁移,可能是通过调节PI3K/Akt通路参与气道重塑,为今后治疗哮喘提供了新思路。
OBJECTIVE: To investigate the effects and mechanisms of HMGB1 stimulation on the proliferation and migration of human airway smooth muscle cells (HASMCs). Methods: HASMCs were cultured in vitro. The purified cells of passage 3 to passage 8 were randomly divided into control group, HMGB1 group (125, 250, 500 and 1 000 ng / m L), TGF-β positive control group ). The proliferation of HASMC was determined by CCK-8 assay. Transwell chamber was used to observe the effect of HMGB1 on HASMC migration. Akt phosphorylation of HASMC was detected by Western blot. Results: Compared with control group, HMGB1 stimulated proliferation of HASMC in a concentration - dependent manner (P <0.05); HMGB1 (500 ng / m L) stimulated HASMC significantly increased cell migration ability compared with control group (P <0.01) Akt phosphorylation of HASMC in 500 ng / m L group was significantly higher than that in control group (P <0.05). Conclusion: HMGB1 can promote the proliferation and migration of HASMC. It may be through the regulation of PI3K / Akt pathway involved in airway remodeling, providing a new idea for the treatment of asthma in the future.