【摘 要】
：
Inter family genetic diversity analysis was undertaken between eight Pinus elliottii × P.caribaea var.hondurensis families using ISSR markers.A total of 480 samples from eight P.elliottii × P.caribaea
【机 构】
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Guangdong Academy of Forestry,Guangzhou,510520,China;Corresponding author
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Inter family genetic diversity analysis was undertaken between eight Pinus elliottii × P.caribaea var.hondurensis families using ISSR markers.A total of 480 samples from eight P.elliottii × P.caribaea families were amplified by using 10 primers.The band number amplified by each primer pair ranged from 2 to 11,with the molecular weight between 250 and 1800bp.Of the 60 discernible fragments generated by 10 selected primers(among 100 primers),30 appeared to be polymorphic.The percentage of polymorphic bands (PPB) was 50% at the families level.and PPB within a families ranged from 3.33% and 23.33%.Genetic diversity index of the families were between 0.0152~0.0872.And Shannon genetic diversity index were between 0.0216 and 0.1294(intra-family index was 0.2934).Analysis showed that only a small proportion(25.65%) of genetic variation resided within families,while a large proportion(74.35%) inter-family genetic differentiation index of Gst=0.7435.According to the genetic distance,we conducted a clustering analysis of the 8 families by UPGMA.Thus,the genetic differentiation among 8 families is relatively higher,with less gene flow (Nm=0.1725).
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