Microfluidic tools for Quantitative Microbiological Studies

来源 :第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术 | 被引量 : 0次 | 上传用户:lixin062
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  Microfluidics have attracted substantial interests in past years for its capacities of high throughput screening and sorting in small volumes,and have been used in a wide range of applications including chemical synthesis,drug discovery,and single cell analysis.1 Herein,we report our recent research projects in using droplet microfluidics and SlipChip technologies2 to solve problems in microbiology.A simple platform for large scale storage of droplets and cultivation of bacterial single cells was developed,and applied to determine the minimum inhibitory concentration(MIC)of bacteria,and cultivation of environmental microorganisms.A SlipChip device for free interface diffusion(FID)based chemotaxis was developed for studying bacterial chemotaxis.A multiplex Dielectrophoresis-PCR SlipChip for rapid detection of pathogens in physiological samples is introduced for point of care diagnostics of infectious diseases.
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