A novel method for improving the efficiency of Agrobacterium-mediated cotyledon node transformation

来源 :第十四届全国植物基因组学大会 | 被引量 : 0次 | 上传用户：ydy611

【摘要】

：

【作者】

：

Bingfu Guo Lijuan Qiu

【机构】

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College of Agronomy,Northeast Agricultural University,Haerbin 150030,China;The National Key Facility

【出处】

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第十四届全国植物基因组学大会

【发表日期】

：

2013年4期

【关键词】

：

soybean ultrasound surfactant Agrobacterium-mediated cotyledon node transformati

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Ultrasonic and surfactant treatments are two methods to increase the efficiency in Agrobacterium-mediated cotyledon node transformation of soybean separately by allowing the access of Agrobacterium to the target cell more easily.In this study, Four cultivated soybeans (Shuilizhan, Tianlong 1, Jack and Zhonghuang 10) were used as receptors to check the transformation efficiency after ultrasonic and surfactant treatments by transforming a vector expressing GUS gene.

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A novel method for improving the efficiency of Agrobacterium-mediated cotyledon node transformation

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