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Objective To study the reasons for misdiagnosis and delayed diagnosis of ectopic pregnancy (EP) and heterotopic pregnancy (HP) conceived after assisted reproductive technology (ART) treatment and to put forward effective preventive measures.Material and Methods Retrospective study of EP (n =180) and HP (n=37) conceived with ART (IVF/ ICSI-ET or FET) from January 2006 to August 2012 at the center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital.Ruptured EP (rEP, n =28) isdefined as either misdiagnosis or delayed diagnosis.