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The inhibition effects of lysozyme,sodium acetate trihydrate and glycine alone on Listeria monocytogenes by measuring the antibacterial rate,The responses surface methodology(RSM) was utilized to optimize L.monocytogenes inhibition affected by using three preservatives.The Box-Behnken design was applied for analyzing coordinated inhibitory effect and confirming the optimum concentration of three preservatives.Results showed that the antimicrobial effectiveness of lysozyme was better than that of sodium acetate trihydrate and glycine.Moreover,there was a significant interactive effect between lysozyme and sodium acetate trihydrate(p<0.05),sodium acetate trihydrate and glycine was found without significant interactive effect(p>0.05).The optimal concentration of lysozyme,sodium acetate trihydrate,glycine was 0.6,4.95,6.44g/L,respectively.At this condition inhibition rate reached the maximum.