【摘 要】
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Background: Promoter hypermethylation leads to altered gene functions and result in malignant cellular transformation.Thus, identification of biomarkers for hypermethylated genes would be useful for e
【机 构】
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Centre of Studies for Preclinical Science Universiti Teknologi MARA Malaysia
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Background: Promoter hypermethylation leads to altered gene functions and result in malignant cellular transformation.Thus, identification of biomarkers for hypermethylated genes would be useful for early diagnosis, prognosis, and therapeutic treatment of oral squamous cell carcinoma (OSCC).Objectives: To screen and validate differentially hypermethylated genes and correlate the hypermethylation-induced silencing genes with demographic and clinic opathologic characteristics of OSCC.Methods: p16, DDAH2 and DUSP1 were screened using microarray and validated by methylation-specific polyrmerase chain reaction (MSPCR) and immunohistochemical (IHC) analysis.Results: The differential hypermethylated genes of p16, DDAH2 and DUSP1 revealed positivity of 78%, 80% and 88% in MSPCR and 30% and 27.5% of immunoreactivity in DDAH2 and DUSP1 respectively.In addition, promoter hypermethylation of p16 gene was found statistically significant association with tumour site of buccal, gum, tongue and lip (P =0.000).Conclusions: Our approaches reveal signature candidates of differentially hypermethylated genes, may possible to become potential biomarkers for OSCC as diagnostic, prognostic and therapeutic targets in the future.
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