Aim: Previous research has shown that luteolin-7-O-glucoside pretreatment had a significant protective effect against doxorubicin-induced cyotoxicity.It could reduce cardiomyocyte apoptosis, intracellular calcium overload, leakage of creatine kinase and lactate dehydrogenase, and reactive oxygen species generation induced by doxorubicin.But the mechanisms are unclear.