【摘 要】
：
Objective usepre-cryosurgery and post-cryosurgery CTCs as tumor biomarkers to evaluate the efficacy of cryosurgery for unresectable hepatocellular carcinoma.Methods use the method of real-time qPCR to
【机 构】
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Fuda Cancer Hospital, Jinan University School of Medicine, Guangzhou, China
【出 处】
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2016第五届国际(广州)癌症治疗论坛暨国际干细胞再生医学学会成立大会
论文部分内容阅读
Objective usepre-cryosurgery and post-cryosurgery CTCs as tumor biomarkers to evaluate the efficacy of cryosurgery for unresectable hepatocellular carcinoma.Methods use the method of real-time qPCR to detect the gene of CTCs related markers and combine magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) to detect the changing number of 47patients with hepatocellular cancer who underwent cryosurgery.Results compare CTCs in 47 patients with hepatocellular cancer before 1 day before cryosurgery, 7 days after cryosurgery, postoperative 30 days after cryosurgery, the numbers of CTCs respectfully are17.70 ± 5.725, 14.64 ±6.761, 10.28 ± 5.598, whichdecreased significantly over with time (P< 0.01).△Ct value of MAGE-3, Survivin and CEA elevated significantly compared with value before cryosurgery;value of 2-△△Ct less than 1, 7 days after treatment 2-△△Ct value of MAGE-3.Survivin and CEA are 0.63 ± 1.56, 0.21 ± 0.22, 0.22 ± 0.34.30 days after treatment 2-△△Ct value of MAGE-3.Survivin and CEA are 0.24 ± 0.82, 0.03 ± 0.07, 0.02 ± 0.08.Whichmeans CTCs related gene expression decreased significantly over with time (P<0.01).Conclusion CTCs as tumor biomarkers can effectively evaluate the efficacy of cryosurgery for unresectable hepatocellular carcinoma.
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