Trace amounts of biogenic amines exist in many foods,but higher levels of them may cause a range of adverse consequences to the eaters,such as headache,nausea,heart palpitations and stomach cramps,even death [1].Certain biogenic amines are used as indicators of freshness of meat.Exploring rapid determination method for biogenic amines is necessary.Microchip electrophoresis is a technique with advantages of low sample consumption and fast analysis speed [2],can be an ideal way for rapid analysis,however,its real application is still rather limited.In this abstract,chip electrophoresis based on low-cost cyclic olefin copolymer (COC) chips and laser induced fluorescence detection (LIF) for the determination of FITC labeled biogenic amines (tryptamine,putrescine,cadaverine) in meat was demonstrated.A series of hydroxyl-group-rich molecules,ethylene glycol,glycerol,polyvinyl alcohol and HPC (hydroxypropyl cellulose) were investigated as buffer additives.The results showed that the molecular weight of the additives was critical; HPC gave the best performance with regard to either separation efficiency or repeatability.Under selected conditions,all biogenic amines could be separated with within 2 min (Figure 1).The limits of detection (S/N = 3) were in the range of 2.47 - 16.8 nM.The linear range (r>0.99) of the calibration curves was upto 5000 nM.Standard deviation of the peak areas and peak times were less than 4.0% and 2.1% respectively.This method has been successfully used for the analysis of tryptamine,putrescine,cadaverine in carp and snakefish samples (Figure 2),the recoveries of standard addition were 83.6%-112.7%.These results show a good prospect of application of the low-cost chip electrophoresis in food safety analysis.