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A dual cell system was used to study the electrogenerative leaching sphalerite-MnO2 under the conditions of presence and absence of Acidithiobacillus thiooxidans (A.thiooxidans).The polarization of anode and cathode, and the relationship between the electric quantity (Q) and some factors, such as the dissolved Zn2+, Fe2+, the time in the bio-electro-generating simultaneous leaching (BEGSL) and electro-generating simultaneous leaching (EGSL), were studied.