【摘 要】
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Floral scent is thought to be an important characteristic to evaluate ornamental flowers,playing a key role in plant ecophysiology.Monoterpenes are an important category of floral scent compounds.In t
【机 构】
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College of Landscape Architecture,Beijing University of Agriculture,Beijing 102206,China
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Floral scent is thought to be an important characteristic to evaluate ornamental flowers,playing a key role in plant ecophysiology.Monoterpenes are an important category of floral scent compounds.In this study,the monoterpene emission from the Lilium siberia flowers was investigated using the automated thermal desorption-gas chromatography/mass spectrometry(ATD-GC/MS)technique.It was found that monoterpenes were the dominant components of floral scent,and showed a significantly developmental emission.The release amounts of monoterpenes first increased and then decreased during the developmental stages,peaking at the full opening stage.In the following study,the first monoterpene gene in Lilium,named LimTPS,was cloned from the flowers.Due to containing conserved sequence DDxxD motif and RRx8W motif,the deduced Li-mTPS was classified into TPS-b monoterpene synthase subfamily.The tissue-specific expression of Li-mTPS revealed the highest level in the outer tepals.Li-mTPS expression showed similar pattern with monoterpene emission except that the peaking time was earlier than monoterpene emission.It was concluded that the Li-mTPS expression played a key role in monoterpene emission from Lilium siberia flowers.These results were helpful to regulate the floral scent of Lilium and breed new varieties.But other regulatory mechanisms also may contribute to the production and emission,which needs to be investigated in the future study.
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