【摘 要】
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[Introduction]Listeria monocytogenes,a food-borne pathogen,can cause listeriosis via contaminated food products.Listeriosis caused by L.monocytogenesmanifests as gastroenteritis,meningitis,mother-to-f
【机 构】
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College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306,China
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[Introduction]Listeria monocytogenes,a food-borne pathogen,can cause listeriosis via contaminated food products.Listeriosis caused by L.monocytogenesmanifests as gastroenteritis,meningitis,mother-to-fetus infections and septicemia.L.monocytogenes WaX12,which isolated from chilled pork,belonged to serotype 1/2a.Because about 95% of human listeriosis cases are caused by serotypes 4b,l/2a,and 1/2b,researching WaX 12 strain is valuable for its character.[Method]: For Illumina pair-end sequencing of WaX12,at least 3μg genomic DNA was used for sequencing library construction.Paired-end libraries with insert sizes of ~300bp were prepared following Illuminas standard genomic DNA library preparation procedure.
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