【摘 要】
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Objective Accumulating evidence supports the effects of miRNA on fatty liver disease.We aimed to investigate miR-122 expression pattern in a steatotic cell model and explore its function.Methods Human
【机 构】
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Guangzhou First Peoples Hospital
【出 处】
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中华医学会第十六次全国病毒性肝炎及肝病学术会议
论文部分内容阅读
Objective Accumulating evidence supports the effects of miRNA on fatty liver disease.We aimed to investigate miR-122 expression pattern in a steatotic cell model and explore its function.Methods Human hepatic cell line (L-02) was treated by oleic acid to establish the steatotic hepatocyte model.Lipid droplets within the cells were observed with laser scanning confocal microscope (LSCM).Triglyceride content of the cells was determined with triglyceride kits.Total RNA was extracted and reversely transcribed into cDNA.The expression ofmiR-122 was measured using qRT-PCR.Afterward, MiR-122 mimic (pre-miR-122) and miR-122inhibitor (anti-miR-122) were transfected into steatotic hepatocytes to observe the changes of.miR-122 expression and lipid content of the cells.
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