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N-ethyl perfluorooctane sulfonamido ethanol(N-EtFOSE),which is a typical precursor of perfluorooctane sulfonate(PFOS),is one of the important potential sources of PFOS to the environment.In this report,bioaccumulation and biotransformation of N-EtFOSE in earthworms(Eisenia fetida)in spiked soil(200 ng·g-1 N-EtFOSE),including depuration rates and metabolism were investigated.