@@ Higher plants have a remarkable ability to regenerate new shoots and/or root even a whole organism from mature tissues direct by cytokinin and auxin in the medium. "Plant regeneration" refers a way for regenerating whole plants via plant somatic embryogenesis and organogenesis. In this process, transcription of them is not simply dependent on DNA-binding transcription factors that turn them on or off, but involves the epigenetic modify. Methylation within the promoter is a critical regulator of gene expression.The methylation status of promoters has a significant role in the regulation of plant development both in vivo and in vitro.