Tissues with tubular structures,such as the trachea,blood vessels,lymph vessels,and intestines are abundant in the bodies of higher animals.[1-2] In this work we describe a general strategy to fabricate tubular structures with multiple types of cells as different layers of the tube walls.By using a stress-induced rolling membrane (SIRM) technique,tubes with controllable sizes were obtained.By rolling,simple patterns on 2D membranes were transformed into complex patterns in 3D tubes (Fig.1).First,different types of cells stained by different dyes were delivered into each channel.When cells attached to the SIRM,the microchannels were removed (Fig.2).When cells on the SIRM grew into confluent or semiconfluent monolayers,the membrane was washed to remove the suspended cells,and one end of the SIRM was cut with a scalpel.The cell-covered SIRM begins to roll up into a tube due to internal stress,and the cell sheet is layered as the tubular wall (Fig.2).To show that a SIRM can allow different layers of cells to communicate with each other,SIRMs were fabricated with porous structures (Fig.3): the top layer is a perforated PDMS membrane,and the bottom layer is a porous membrane fabricated by electrospinning (ES).Cells were cultured on this kind of SIRM (Fig.3),and cells in each layer could communicate with each other even after rolling up,because of the porous structure.In conclusion,we present a strategy to fabricate tubular structures with layered walls made of multiple types of orientated cells,which circumvents the principal structural limitations for tubular tissue biomimicry.This method may be widely used for simulation of many tubular tissues.