【摘 要】
：
Myopalladin (MYPN) is a multifunctional protein whose main role is to maintain sarcomeric integrity and regulate Z-line structure.It is an important candidate gene for meat quality selection through m
【机 构】
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College of Animal Science and Technology, Northwest A&F University,Yangling, Shaanxi, P.R.China
【出 处】
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2011中国肉牛选育改良与产业发展国际研讨会
论文部分内容阅读
Myopalladin (MYPN) is a multifunctional protein whose main role is to maintain sarcomeric integrity and regulate Z-line structure.It is an important candidate gene for meat quality selection through marker-assisted selection (MAS).In this study, based on PCR-RFLP technology, we discovered one single nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene.Allele frequencies of the SNP were investigated and evaluated by the X2 test in 660 cattle populations, and only the Nanyang population was not in Hardy-Weinberg equilibrium.Gene heterozygosity, effective allele numbers and PIC (polymorphism information content) of the bovine MYPN locus in seven populations varied from 0.3888 to 0.4998, 1.6360 to 1.9992, 0.3132 to 0.3749, respectively.We also evaluated the potential association of the SNP with ultrasound traits in 399 individuals, and there was a significant effect on the ultrasound loin muscle area (ULMA) in the populations tested (P<0.05).Furthermore, meat quality traits were analyzed in another 61 Qinchuan individuals by the comparison of the genotypes with regard to their phenotypic data.The results show that animals with the genotype GG had higher mean values for loin-eye area (LEA) (P<0.05) and water holding capacity (WHC)(P<0.01) than those with AA or AG genotypes.These results suggest that the SNP of the MYPN gene could be a very useful genetic marker for meat quality traits in cattle reproduction and breeding.
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