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Lilies are commercially important flower bulbs cultivated mainly in Asia,North America and Europe as a cut flower,pot and garden plants.Production of polyploid and crosses using the polyploid in lily breeding program will be useful to produce novel cultivars.The present study was undertaken to produce tetraploid plants by in vitro chromosome doubling and to obtain triploid plants by subsequent crosses with tetraploid and diploid plants in Lilium×formolongi White Aga.