Flow cytometry and cell separation are critical technologies that have played important roles in cell biology studies and biomedical diagnostics since their inception over half a century ago.Recent developments in microfluidics offer advantages in many aspects and new functions to this established technology, which dovetail with the contemporary interest in the behavior of small cell populations or even single cells in controlled microenvironments.1 In this report, we have developed a microfluidic platform, composed of a separation and a characterization circuit, to achieve simultaneous on-chip cell separation and characterization.The microfluidic separation circuit contains a sharp insulating cusp aimed at inducing DEP and deflecting cells by size, while the characterization circuit behind the separation one consists of a pair of MOSFET-based resistive pulse sensors in order to size the separated cells and define the separation performance.