目的:以微流控芯片为平台,以激光诱导荧光为检测手段,旨在探索AGT基因[G(-6)A]位点基因多态性在沈阳地区人群中的分布及与原发性高血压的关联,并为大规模人群基因多态性分析提供一高效、快速和灵敏的检测技术。方法:采用聚合酶链反应—限制性片段长度多态性(PCR-RELP)的方法,分别运用微流控芯片电泳与琼脂糖凝胶电泳对沈阳地区汉族人群103例正常人与123例高血压患者AGT基因(-6)位点的多态性进行分析。结果:(1)微流控芯片能在250s内对AGT基因PCR产物酶切片段进行芯片电泳分析;(2)沈阳地区正常对照组与高血压组中AGT基因(-6)位点A等位基因均有较高的发生频率(0.7038,0.7073),突变位点多态性无统计学差异(χ2=0.024,P>0.05)。结论:(1)微流控芯片同琼脂糖凝胶电泳相比,分析速度比凝胶电泳快,耗样量少,检测灵敏度高;(2)沈阳地区汉族人群中AGT基因核心启动子区域-6位点等位基因A有较高的分布频率,但G/A的基因变异EH发病不相关联,此位点可能仅仅是一个基因多态性标志。 OBJECTIVE: To explore the distribution of AGT gene G (-6) A] polymorphism in Shenyang population and its relationship with primary hypermethylation by using microfluidic chip as platform and laser-induced fluorescence as detection method Blood pressure and provide an efficient, rapid and sensitive detection technique for gene polymorphism analysis of large-scale population. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) method was used to analyze the relationship between 103 healthy controls and 123 hypertensive subjects in Shenyang Han population, using microfluidic chip electrophoresis and agarose gel electrophoresis Patients with AGT gene (-6) loci polymorphisms were analyzed. Results: (1) Microfluidic chips could perform chip electrophoresis analysis of AGT gene PCR products within 250s; (2) A allele of AGT gene (-6) in normal control group and hypertension group The gene frequencies were all higher (0.7038,0.7073). There was no significant difference in the polymorphism between the two groups (χ2 = 0.024, P> 0.05). Conclusion: (1) Compared with agarose gel electrophoresis, microfluidic chip has faster analysis speed than gel electrophoresis, less sample consumption and high detection sensitivity; (2) AGT gene core promoter region in Shenyang Han population - There was a higher distribution frequency of allele A in 6 loci, but the genetic variation of G / A was not correlated with the incidence of EH. This locus may be only a genetic polymorphism marker.