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The most important indoor allergens for humans are house dust mites(HDM).Fourteen Dermatophagoides farinae allergens(Der f 1-3,6,7,10,11,13-18,and 22)are reported although more than 30 allergens have been estimated in D.farinae.Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D.farina extracts.