骨髓间充质干细胞在肝癌前病变大鼠肝脏内的分化及中药槲芪散的干预作用

来源 :第八届中国病理生理学会消化专业委员会学术会议 | 被引量 : 0次 | 上传用户:haschie
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  目的:探讨骨髓源性肝脏干细胞是否参与肝癌前病变的形成,评价方剂槲芪散对肝癌前病变的干预作用。方法:取80g体重的雄性Wistar大鼠的股骨和胫骨,用PBS冲洗骨髓,按密度梯度离心法原代分离培养骨髓间充质干细胞(mesenchymal stem cell,MSCs)。用绿色荧光蛋白(GFP)作为追踪标记物将MSCs进行标记。将大鼠分为6组:即常规饲养组、模型组、模型+MSCs移植组、肝大部切除+MSCs移植组、槲芪散治疗组8 g/(kg·d)、槲寄生总碱治疗组8g/(kg·d)。
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