Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome

来源 :2013国际猪繁殖与呼吸综合征学术研讨会 | 被引量 : 0次 | 上传用户:qq2285387
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The high level of diversity among PRRS viruses makes it very important to monitor the overall genetic variations in relation to the sensitivity of diagnostic tests and vaccination efficacy,but only few full genome sequences of PRRSV strains isolated in Europe have been made public available.In the present study,fast and robust methods for long range RT-PCR amplification and subsequent next generation sequencing (NGS) of PRRSV Type 1 and Type 2 viruses were developed and validated on nine Type 1 and nine Type 2 PRRSV viruses.The methods were shown to generate robust and reliable sequences both on primary material and cell culture adapted viruses and the protocols were shown to perform well on all three NGS platforms tested (Roche 454 FLX,Illumina HiSeq 2000,and Ion Torrent PGMTM Sequencer),
其他文献
Porcine circovirus associated disease (PCVAD) describes a group of complex syndromes such as porcine multi-systemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS).The c
会议
Introduction:The neutralizing antibody (nAb) response following PRRSV infection is generally described as weak and delayed.Furthermore,when present,nAb is primarily directed against only the homologou
会议
Highly pathogenic PRRS (HP-PRRS) is one of the most economically significant viral diseases,causing great losses in China and continuing to be a threat for the swine industry.The glycosylated protein
会议
Porcine reproductive and respiratory syndrome virus (PRRSV) is mainly responsible for the heavy economic losses in pig industry in the world.Current vaccination strategies provide only a limited prote
会议
Objective:Porcine reproductive and respiratory syndrome (PRRS) viruses are recognized as possessing a high degree of variability,and different strains have various Pathogenicity of the host.To investi
会议
Type I IFN(IFN-alpha and IFN-beta) is the first responder against animal virus infections.When virus infects,the virus could be recognized by the pathogen-associated molecular pattern receptors and le
会议
Objectives:PRRS is one of the most economically significant diseases of swine.Vaccination is one strategy used to control PRRS.However,the current vaccines do not provide adequate long term protection
会议
Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of porcine reproductive and respiratory syndrome,which continues to affect the Chinese swine industry and emergency o
会议
Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) can cause respiratory tract disease,death,sow abortion and serious immunosuppression.It is one of the most economically importa
会议
Porcine reproductive and respiratory syndrome viruses (PRRSV) have been divided into Type 1 (European) and Type 2 (North American) viruses.PRRSV are very diverse and Type 1 viruses have even been furt
会议