【摘 要】
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OBJECTIVE To study the protective effects of exogenous phosphocreatine (CP) on brain injury after cardiopulmonary resuscitation (CPR) in rats.METHODS one handrad and sixty adult male SD rats were rand
【机 构】
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Department of Emergency Medicine of The First Affiliated Hospital of Wenzhou Medical University
【出 处】
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中华医学会急诊医学分会第十八次全国急诊医学学术年会
论文部分内容阅读
OBJECTIVE To study the protective effects of exogenous phosphocreatine (CP) on brain injury after cardiopulmonary resuscitation (CPR) in rats.METHODS one handrad and sixty adult male SD rats were randomly divided into 4 groups: control group (group A),CPR group (group B),low-dose CP group (group C,CP0.5g/kg at beginning of ROSC),high-dose CP group (group C, CP1g/kg at beginning of ROSC),Asphyxiation cardiac arrest and CPR model was used in the study.Brain were taken at 0.5h, 3h, 6h, 12h, 24h.The contents of adenosine triphosphate(ATP), adenosjne diphosphate(ADP) and adenosine onophosphate(AMP)in the brain tissue were measured by HPLC, and the values of total denine nucleolldes(TAN) and energy charge(EC) were calculated;the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in brain tissue were assayed by spectrophotometric method.The pathological changes of brain were observed under optical microscope.
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