外源激素诱导对F1代2+圆斑星鲽雌鱼性腺发育成熟的影响

来源 :第一届全国鲆鲽类产业可持续发展研讨会 | 被引量 : 0次 | 上传用户：NewComeMan

【摘要】

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【作者】

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徐永江柳学周赵明

【机构】

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中国水产科学研究院黄海水产研究所,青岛,266071 中国水产科学研究院黄海水产研究所,青岛,26

【出处】

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第一届全国鲆鲽类产业可持续发展研讨会

【发表日期】

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2010年10期

【关键词】

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圆斑星鲽性腺发育激素诱导表达调控

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　　采用外源激素诱导方法,研究了外源激素sGnRH、LHRHa诱导2+龄雌性圆斑星鲽后备亲鱼性腺发育的效果。结果表明,2+龄圆斑星鲽对外源激素的诱导应答效应较性成熟亲鱼弱,卵母细胞可达到5时相早中期,但未能发生水合作用而成熟。两种外源激素以sGnRH诱导效果为好。外源激素诱导后,血浆中性类固醇激素(雌二醇和睾酮)的表达水平显示显著升高,96h后表达水平下降恢复至正常水平。同时，肝细胞道怪增加，有约30%的亲鱼性腺可见相对明显的隆起。本研究未能获得亲鱼产卵，其可能是因为2+亲鱼内分泌成熟调控系统对外源激素的响应机制未完全建立。本结果为认识人工养殖条件下圆斑星鲽的生殖特性和其周年受精卵获取提供了理论支持和技术依据。

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外源激素诱导对F1代2+圆斑星鲽雌鱼性腺发育成熟的影响

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