【摘 要】
：
Porcine circovirus type 2 (PCV2) is a major causal agent of postweaning multisystemic wasting syndrome (PMWS) in piglets.To investigate the feasibility of P
【机 构】
：
HarbinVeterinaryResearchInstitute,TheChineseAcademyofAgriculturalSciences,Harbin,China
论文部分内容阅读
Porcine circovirus type 2 (PCV2) is a major causal agent of postweaning multisystemic wasting syndrome (PMWS) in piglets.To investigate the feasibility of PCV2 expressing an exogenous epitope, a 14 amino acid V5 epitope derived from simian parainfluenza virus type 5, was inserted into the C-terminus of the capsid protein.Recombinant PCV2 expressing the V5 epitope, recPCV2/CL-V5, was rescued by transfecting an infectious clone into PK-15 cells and was characterised by an immunoperoxidase monolayer assay (IPMA), a serum neutralisation assay (SNA), a capture enzyme-linked immunosorbent assay (ELISA) and immune electron microscopy.
其他文献
It had been reported that human immunodeficiency virus type 1 (HIV-1) encodes at least three viral microRNAs (miRNAs).Although the cellular miRNAs have been
Untranslated regions (UTRs) of influenza virus are considered to possess plural functions, composed ofpolyadenylation and signals for genomic packaging and
目的 评价新生儿接种5微克(μg)重组乙型肝炎(乙肝)疫苗(酿酒酵母)[Hepatitis B Vaccine(HepB) Made by Recombinant Deoxyribonucleic Acid Techniques in Saccharomyces Ce
MicroRNAs (miRNAs)是一类可以通过转录后调控来调控基因表达的非编码小RNA.越来越多的研究证明miRNA在调控宿主-病原体相互作用和固有免疫中起着重要的作用.在本研究中,
背景:乙型肝炎病毒(Hepatitis B Virus,HBV)感染是全球范围影响健康的重要问题,慢性感染人群存在肝硬化和肝细胞癌的高患病风险.HBV感染具有宿主特异性,在自然情况下,HBV不感
5%-25% of HIV-1 infected individuals produce broad neutralizing antibodies (bNAb).A HIV-1 vaccine (RV144) has shown a protection partially associated with e
病毒常通过各种机制抑制宿主细胞凋亡,从而促进病毒复制。博尔纳病病毒(Borna disease virus,BDV)是一种嗜神经病毒,感染宿主后常建立起非细胞病变性持续感染。虽然目前
Background: Porcine circovirus type 2 (PCV2) is considered to be the primary causative agent of postweaningmultisystemic wasting syndrome (PMWS), which has beco
Background:Superinfection resistance (SIR) results from interactions between viruses and target cells.A better understanding of the mechanisms that determine th
Background: The capsid (Cap) protein of PCV2 is the major immunogenic protein that is crucial to induce PCV2-specific neutralizing antibodies and protective imm