论文部分内容阅读
Buyang Huanwu Decoction (BYHWD, 补阳还五汤) is a traditional Chinese prescription that has been used for hundreds of years to treat paralysis, and for the treatment of coronary heart disease (CHD) with qi deficiency and blood stasis syndrome.Its effect on antioxidant and drug-metabolizing enzymes are unknown.To assess the interactions of herb and drug metabolism, effects of BYHWD on antioxidant enzymes and drug-metabolizing enzymes were studied in rats.Treatment with BYHWD at the dosage of 6.42, 12.83 or 25.66g/kg per day for 15 days, microsomes and cytosols isolated from liver tissues were prepared, the activities of ALT, AST, Hepatosomatic Index and antioxidant enzymes in serum were not significantly affected.In cytosols, the activities of CAT was significantly increased at the dosage of 12.83g/kg, and all the other antioxidant activities and MDA levels were not affected by this treatment.BYHWD had no effect on cytochrome b5, NADPH-cytochrome P450 reductase, CYP3A and UGT.At the highest dose (25.66g/kg), the activities of CYP2E1 were significantly inhibited, and the activities of GST and the level of GSH were increased.These results demonstrated that BYHWD have detoxification and antioxidant functions, and patients should be cautioned about the herb-drug interaction of BYHWD and CYP2E1 substrates.