Accompanied by industrial globalization,rapid urbanization,and population increment,mass production and staple trading for food consumption is upsoaring continuously,foodborne disease resulted from various food safety issues is currently an crucial public health concern worldwide,which has not only created a great burden on both economy and society,but also greatly threatened the sustainability of mankind livelihood and human reproduction.In order to better ensure food safety and thus effectively curb the occurrence of foodborne diseases,the development and evolving of inspection strategies are indispensable measures for quality assurance and conformity assessment.Nowadays,as complementary measures to classic analytical methods,highly sensitive microspheres-based microfluidic immunoassays have found their increasingly important roles in various domains of food analysis,and proteins immobilized on microspheres surface is one of the important steps of microfluidic immunological technologies.This presentation mainly introduces the detection methods and quantification of protein on the surface of microspheres for microfluidic immunological food safety detection,and also explores the effects of different initially added protein concentrations and different blending times on quantification.