Cultivated peanut or groundnut (Arachis hypogaea L.) is an important oilseed crop with an allotetraploid genome (AABB, 2n =4x =40).The marker density, however, is not very satisfactory especially in the population-specific linkage map.Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variation existing in genome.However, until recently only sporadic SNP markers developed, informative SNP markers in cultivated peanuts have not been reported yet.We constructed reduced representation libraries (RRLs) for two groundnut inbred lines Zhonghua 5 and ICGV86699, and with their 166 RIL progenies using ddRADseq technique.Using multiple SNP prediction methods to discover and genotype SNPs, a total of 1 725 predicted SNPs were identified.An intraspecific linkage map was constructed and comprised of 1 625 marker loci including 1570 SNPs and 55 previously published simple sequence repeat (SSR) loci distributed on 20 LGs (a01 to a10 and b01 to b10) spanning a map distance of 1641.7 cM with an average map density of 1.2 cM.By using Sanger sequencing method, the validation rate of these SNPs reached 94%.We have demonstrated how next generation sequencing was combined with high-throughput SNP detection assays to quickly discover large numbers of SNPs in cultivated peanut.The first high-density, SNP based linkage map for A.hypogaea was generated that can serve as a reference map for cultivated Arachis species.Our finding will enrich the current molecular marker resources and contribute to enhance our understanding of the cultivated tetraploid peanut genome.