【摘 要】
：
溶血磷脂酸酰基转移酶(LPAT)是植物油脂合成途径的关键酶,负责催化溶血磷脂酸(LPA)生成磷脂酸(PA)的反应.植物LPAT的编码基因是提高油料作物含油量和改良油脂组成的重要候选基因.本研究通过构建花生种子cDNA文库,采用RACE技术,克隆了花生AhLPA T4基因.该基因全长1631bp,编码一条383个氨基酸组成的多肽,预测分子量为43.8kD.AhLPAT4蛋白含有一个典型的酰基转移酶保
【机 构】
：
中国农业科学院油料作物研究所,湖北武汉,430062;河北省农林科学院粮油作物研究所,河北石家庄,050035
【出 处】
：
中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
溶血磷脂酸酰基转移酶(LPAT)是植物油脂合成途径的关键酶,负责催化溶血磷脂酸(LPA)生成磷脂酸(PA)的反应.植物LPAT的编码基因是提高油料作物含油量和改良油脂组成的重要候选基因.本研究通过构建花生种子cDNA文库,采用RACE技术,克隆了花生AhLPA T4基因.该基因全长1631bp,编码一条383个氨基酸组成的多肽,预测分子量为43.8kD.AhLPAT4蛋白含有一个典型的酰基转移酶保守结构域和4个高度保守的基序(Ⅰ-Ⅳ),并含有乙酰辅酶A催化位点和酰基绑定位点.生物信息学分析表明,AhLPAT4含有4个跨膜结构域.预测表明,AhLPAT4定位于内质网膜上,其中保守基序Ⅰ和基序Ⅱ、Ⅲ分别被跨膜结构分隔在膜的两侧,即分别位于胞质和内质网腔内.AhLPAT4蛋白表面疏水残基折叠形成疏水通道,可以促进酰基与磷酸基团的结合.亚细胞定位分析表明,AhLPAT4属于细胞质蛋白.进化树分析表明,AhLPAT4与大豆、拟南芥和蓖麻的同源LPAT4蛋白氨基酸序列的同源性为63.7％～78.3％.AhLPAT4基因在除花以外的其他器官中普遍表达,花生种子不同发育阶段AhLPAT4的表达与种子油脂积累过程并不一致.
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