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Objective: To investigate the mechanism of androgen replacement therapy (ART) improving erectile dysfunction (ED) in castrated rats.Methods: We randomly divided 40 8-week-old healthy male SD rats into 4 groups: group A was the control,and rats of the group B,C and D were castrated,then rats in the groups C and D were treated with different concentrations of testosterone undecanoate orally every day (C: 10 mg/kg,D: 20 mg/kg),while other groups with 0.9% NS instead.