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P-Le was extracted from Lentinula edodes fruitbody by hot water,precipated by ethanol,deproteinized by sevage method,the optimal conditions of removal protein for P-Le by orthogonal test:the proportion between sample and sevage agent was 1∶1,the proportion between chloroform and orphobutyl alcohol was 4∶1,the time of abstraction was 30 minutes,and the deproteinization rate is up to 26.32%.