【摘 要】
：
Automated patch-clamp has become common in drug development and safety programs because it enables efficient and systematic testing of compounds against ion channels during voltage-clamp.In neuroscien
【机 构】
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Sophion Bioscience A/S,Biolinscientific AB
【出 处】
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第九届海内外华人神经科学家研讨会(The 9th Symposium for Chinese Neuroscientis
论文部分内容阅读
Automated patch-clamp has become common in drug development and safety programs because it enables efficient and systematic testing of compounds against ion channels during voltage-clamp.In neuroscience research the conventional patch clamp technique has been favored,but refining of the automated patch-clamp techniques providing high quality data makes more widely used in areas of ion channel studies and neuroscience research has been accelerated due to the higher through-put of the automated systems.Here,we show the wider potential of automated patch-clamp,by showing data recording on primary astrocytes and microglia cells generated from rat brain on the automated giga-seal patchclamp system QPatch from Sophion.Under automated control,giga-seals and whole-cell access are achieved followed by preprogrammed routines of voltage protocols and fast extracellular solution exchange.On the primary microglia we observed what is possible a small-conductance Ca2+-activated K+channels that could be activated by NS309,an opener of small and intermediate conductance Ca2+-activated K+channels.We also recorded inward rectifier K+channels and voltage-gated K+channels that could be blocked by 3 uM quinidine.On the primary astrocytes we recorded voltage-gated K+channels that could be blocked quinidine and in addition we found a Bz-ATP activated current that could correspond to a ligand-gated P2X receptor.GABA receptors were also observed.Automated patch-clamping has become a powerful method for investigating the function and regulation of ionic channels of primary cells.
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