论文部分内容阅读
Background Human genome project(HGP) has unveiled the primary sequence of all three billion bases of the human genome.Biochip,which of using an ordered array of biomolecules on a chip to simultaneously examine lots of biochemical samples emerges as the times reguire following the development of HGP.Functional techniques,such as cDNA microarrays enable analysis of expression levels of thousands of genes at once.It is a challenging task to validate,prioritize and select the best targets from tens of thousands of candidate genes and proteins.Analysis of the molecular targets in situ at the cellular level,assessment of their expression across all tissues and diseases and evaluation of their clinical significance would provide significant additional information to target selection.Analysis of 300 molecular targets corresponds to a mere 0.75% of all of the estimated 40 000 genes in the human genome.This indicates how genome-scale research will not be possible using conventional molecular pathology techniques.