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目的验证重组胰高血糖素样多肽2(GLP-2)对严重烧伤大鼠的肠道保护作用。方法将SD大鼠随机分为正常对照组;烧伤对照组;重组GLP-2治疗组(重组治疗组),烧伤后4 h皮下注射重组GLP-2,100 nmol·kg~(-1)·d~(-1);化学合成GLP-2治疗组(合成治疗组),烧伤4 h后皮下注射合成GLP-2,剂量同上。每组6只大鼠。伤后第7天检测各致伤组大鼠肠黏膜通透性、肠黏膜湿质量与肠段及躯壳质量比、肠黏膜蛋白含量以及观察肠道组织病理学变化,正常对照组观察指标相同。结果重组治疗组及合成治疗组与烧伤对照组[(0.350±0.040)mg/ml]比较,大鼠肠黏膜通透性明显降低(P<0.01),分别为(0.250±0.026)、(0.243±0.008)mg/ml;肠黏膜湿质量与躯壳质量比及肠黏膜蛋白含量明显增加,重组治疗组大鼠肠黏膜蛋白含量为(57.9±2.8)mg/g,高于合成治疗组(48.9±4.1)mg/g。与正常对照组比较,各致伤组大鼠伤后第7天肠黏膜绒毛明显变短脱落、排列紊乱、基底层变薄。重组治疗组损伤较烧伤对照组有所减轻,与合成治疗组大鼠无明显区别。结论重组GLP-2与合成GLP-2,能减轻烧伤大鼠肠道损伤,具有明显的肠道保护作用。
Objective To verify the protective effect of recombinant human glucagon-like polypeptide 2 (GLP-2) on the gut in severely burned rats. Methods SD rats were randomly divided into normal control group, burn control group, recombinant GLP-2 treatment group (recombinant therapy group), and subcutaneous injection of recombinant GLP-2 at 100 micromol · kg -1 (-1) d ~ -1). The GLP-2-treated group (synthetic treatment group) was chemically synthesized, and GLP-2 was injected subcutaneously 4 h after burn. The dose was the same as above. 6 rats in each group. On the 7th day after injury, the intestinal mucosal permeability, the intestinal mucosa mass ratio, the intestinal mucosa mass ratio, the intestinal mucosa protein content and the intestinal pathological changes were observed in the rats of each injury group. The observation indexes of the normal control group were the same. Results Compared with the burn control group ([(0.350 ± 0.040) mg / ml], the intestinal mucosal permeability of the treated group and the untreated group were significantly decreased (P <0.01) 0.008) mg / ml. Intestinal mucosa wet weight to body mass ratio and intestinal mucosa protein content were significantly increased. Intestinal mucosal protein content in the recombinant treatment group was (57.9 ± 2.8) mg / g, higher than that in the synthetic treatment group (48.9 ± 4.1 ) mg / g. Compared with the normal control group, the intestinal mucosa villi of the rats in each injury group became shorter, shedding, disordered and the basal layer became thinner on the 7th day after injury. Compared with the burn control group, the damage of the recombinant treatment group was alleviated, and there was no significant difference between the treatment group and the synthetic treatment group. Conclusion Recombinant GLP-2 and GLP-2 can relieve intestinal injury in burn rats and have obvious intestinal protective effect.