An increasing number of antibodies and their validation are promising candidates for developing novel strategy for the life science.Such assays have a potential complement to gene expression analyzed by comprehensive assays such as DNA microarrays and conventional proteomics approaches.Antibody microarrays are thought to represent those functional assays.While a variety of attempts have been made to apply DNA microarray technology to antibody microarrays,an optimized protocol has not yet been completely established.We have been conductinga project to comprehensively generate antibodies against mouse KIAA proteins (mKIAA) and human transcriptional factors.