切割PLRV复制酶基因负链的核酶基因对马铃薯转化及检测

被引量 : 4次 | 上传用户:cdzq911
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
本文将已分别构建到双元载体质粒 pROK Ⅱ中特异切割马铃薯卷叶病毒(PLRV)复制酶基因负链的单切点和双切点双体核酶基因所得重组质粒pRRz和pRDRz通过三亲融合法分别转入根癌农杆菌LBA4404中,单切点核酶转化了马铃薯栽培品种Desiree和乌盟601并获得抗性小植株,双切点双体核酶转化了Desiree并获得抗性小植株,经PCR检测证明核酶基因己整合到马铃薯基因组中,Norther
其他文献
本文选用野生种福州金山割手密、中国种松溪竹蔗、热带种Badila和栽培品种新台糖15、闽糖70/611等5种甘蔗基因型作为供试材料。选用甘蔗幼叶诱导形成愈伤组织,继代培养后,挑选胚性愈伤组织进行悬浮培养,将培养物中小而圆的细胞富集,用于分离原生质体。进行了纤维素酶浓度、酶解时间、甘露醇预处理对甘蔗悬浮细胞原生质体分离的影响研究;比较不同甘蔗基因型间以及同一材料主茎与分蘖茎间原生质体分离和培养...
This paper investigates the secrecy performance of maximal ratio combining (MRC) and selection combining (SC) with imperfect channel state information (CSI) in
The current boom in the Inteet of Things (IoT) is changing daily life in many ways, from wearable devices to con-nected vehicles and smart cities. We used to re
The Competition for Authenticated Encryption: Security, Applicability, and Robustness (CAESAR) supported by the National Institute of Standards and Technology (
The evolutionary algorithm, a subset of computational intelligence techniques, is a generic population-based stochastic optimization algorithm which uses a mech