小麦WK蛋白激酶互作候选蛋白的初步验证及其分子生物学特性分析

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高等植物在生长过程中经常受到干旱、高温、低温、高盐等非生物的胁迫,植物能够快速感知这些不良环境的变化且主动适应,而适应的过程就涉及到细胞内与细胞间的信号传递。蛋白质的磷酸化和去磷酸化是生物体内能量代谢以及信号传递中的重要生化反应,而蛋白质的磷酸化主要是在蛋白激酶的催化下完成的。对小麦蛋白激酶的研究将为小麦的生理代谢和耐胁迫性的机制提供有利的依据。本实验以小麦中蛋白激酶WK为诱饵通过酵母双杂交筛选得到WD40和MAPK3蛋白,主要验证WK与WD40和MAPK3之间的相互作用,并对WD40和MAPK3分
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