小麦成熟胚再生体系优化和ppo-RNAi构件的遗传转化

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【摘要】

：

面粉白度是衡量小麦品质的重要性状之一，受遗传和环境影响。其中，多酚氧化酶（polyphenol oxidase，PPO）是引起面条(团)颜色褐变的主要原因。研究表明降低籽粒PPO活性可以改良小麦面粉白度，因此可以通过基因转化、RNAi等基因工程方法来降低内源PPO基因的表达，抑制PPO的活性，增加面粉白度等。本研究首先进行了小麦成熟胚再生体系的优化和优良基因型的筛选，其次利用ppo基因构建了植物R

【作者】

：

陈俊男

【机构】

：

山东农业大学

【发表日期】

：

2012年06期

【关键词】

：

小麦体系优化面粉白度 RNAi 多酚氧化酶筛选

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小麦成熟胚再生体系优化和ppo-RNAi构件的遗传转化

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