甘蓝型油菜早熟基因遗传转化体系的建立及转基因植株初步鉴定

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早熟是油菜育种改良的目标性状之一。近年来,随着现代生物技术的飞速发展与成熟,农杆菌介导的转基因技术广泛地运用在油菜上,但转化效率受到众多因素的影响,因此建立和完善一套稳定高效的再生和遗传转化体系非常重要。本文以甘蓝型油菜品种‘浙双758’等为研究材料,探讨了油菜再生和遗传转化体系中的相关问题,并对转基因苗进行了初步的PCR鉴定,取得的主要研究结果如下:1.以子叶柄为外植体油菜再生体系的改进与完善。在本试验室研究基础上,进一步研究了切下子叶柄外植体时油菜的苗龄,常用外源激素的浓度及配比组合对子
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