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钼酸钠(M_0)能稳定受体对甾体的结合活力、并抑制其降解和减少其结构的多相性。在有M_0存在的缓冲系统中,通过对人类乳腺癌、良性和恶性的子宫内膜细胞溶质雌激素受体(ER)和孕激素受体(PR)进行梯度超离心和琼脂糖凝胶过滤的研究,得到的结果为:受体的沉降系数(S_(20,w))的平均值为9.7±0.3S(n=31),其流体力学半径(R_(?))的平均值为75±4(?)(n=22);综合超离心和凝胶过滤的分析资料计算得到,M_0稳定时受体分子量(M)的平均值为304,000,轴比(a/b)=10。本文报告了47例人乳腺肿瘤、10例恶性子宫内膜、10例良性子宫内膜的细胞溶质ER 和PR 的浓度及受体阳性、阴性及可疑的分布。乳腺肿瘤的ER 阳性率为48%,PR 阳性率为72%(>3fmol/mg 蛋白为阳性),90%恶性子宫内膜的PR 为阳性;100%良性子宫内膜的ER 或PR 均为阳性。绝经前妇女的良性子宫内膜的PR 浓度最高,为458±275fmol/mg 蛋白。对24例标本均测定了解离常数(K_d),其中乳腺和恶性子宫内膜组织中PR-[~3H]R5020复合物的K_d 平均值为2-3nM,乳腺癌组织中ER-[~3H]E_2复合物的K_d 平均值为0.4nM。
Sodium molybdate (M_0) can stabilize the binding activity of the receptor to steroids and inhibit its degradation and reduce the heterogeneity of its structure. In a buffered system with M_0, mice were immunized by gradient ultracentrifugation and agarose gel filtration on human breast cancer, the benign and malignant endometrial cytosolic estrogen receptor (ER) and progesterone receptor (PR) The results showed that the mean value of the sedimentation coefficient (S_ (20, w)) of the receptor was 9.7 ± 0.3S (n = 31) and the mean value of the hydrodynamic radius (R_ (?)) Was 75 ± 4 (?) (N = 22). The calculated values of the molecular weight (M) of the receptor at M0 stability were 304,000 and the axial ratio (a / b) = 10 as calculated from the data of the analysis by ultracentrifugation and gel filtration. This paper reports the concentration and receptor positive, negative and suspicious distribution of cytosolic ER and PR in 47 cases of human breast cancer, 10 cases of malignant endometrium and 10 cases of benign endometrium. ER positive rate of breast cancer was 48%, PR positive rate was 72% (> 3fmol / mg protein is positive), 90% of malignant endometrial PR positive; 100% of benign endometrial ER or PR were positive . The pre-menopausal women had the highest PR concentration of benign endometrium, which was 458 ± 275 fmol / mg protein. The dissociation constants (K_d) were determined in all 24 specimens. The mean K_d of PR- [~ 3H] R5020 complex in breast and malignant endometrial tissues was 2-3nM, and ER- [~ 3H] in breast cancer tissues The average K_d of E_2 complex was 0.4 nM.