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目的 探讨用免疫印迹发光法 ,检测乳腺肿瘤患者血清细胞质胸苷激酶 (TK1)的研究及临床应用。方法 利用抗胸苷激酶单克隆抗体 (anti TK1mAb)建立点免疫印迹发光法 ,分析乳腺肿瘤患者、非肿瘤患者和体检健康者血清TK1的水平。结果 术前的乳腺癌患者、乳腺良性肿瘤患者、非肿瘤患者和体检健康者血清TK1的水平分别为 (39.3± 33.9) ,(3.7± 3.9) ,(1.7± 0 .5 )和 (1.4±0 .5 )pmol/L。淋巴结转移的术前和术后乳腺癌患者、乳腺良性肿瘤患者血清TK1与体检健康者差异有显著意义 (P <0 .0 0 1,P <0 .0 0 1,P <0 .0 5 ) ;而淋巴结未转移的术后乳腺癌患者、非肿瘤患者血清TK1与体检健康者差异无显著意义 (P >0 .10 ,P >0 .0 5 )。通过对术后乳腺癌患者血清TK1检测发现 ,淋巴结未转移患者TK1水平明显低于淋巴结转移患者 (P <0 .0 0 1)。结论 该方法有较好的敏感性与特异性 ,用于血清TK1的检测可作为乳腺癌的辅助诊断、术后疗效观察和预后判断的方法之一
Objective To investigate the study and clinical application of serum thymidine kinase (TK1) in patients with breast tumors by immunoblot luminescence. Methods The anti-thymidine kinase monoclonal antibody (anti TK1 mAb) was used to establish dot immunoblot luminescence. The levels of serum TK1 in breast cancer patients, non-tumor patients and healthy persons were analyzed. Results The levels of serum TK1 in preoperative breast cancer patients, benign breast tumor patients, non-tumor patients, and healthy subjects were (39.3±33.9), (3.7± 3.9), (1.7±0.5), and (1.4±0) respectively. .5) pmol/L. There was a significant difference in serum TK1 levels between preoperative and postoperative breast cancer patients and benign breast cancer patients with lymph node metastasis (P < 0.01, P <0.01, P <0.05). However, there was no significant difference in serum TK1 levels between postoperative breast cancer patients and non-cancer patients with non-metastatic lymph nodes (P > 0.10, P > 0.05). The detection of serum TK1 in patients with breast cancer after surgery found that the level of TK1 in patients with no lymph node metastasis was significantly lower than that in patients with lymph node metastasis (P < 0.01). Conclusion This method has good sensitivity and specificity. The detection of serum TK1 can be used as one of the methods of assisted diagnosis, postoperative curative effect observation and prognosis judgment of breast cancer.