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采用磷脂脂肪酸(PLFA)分析方法,对山西长治襄垣五阳煤矿采煤沉陷区复垦土壤的微生物群落结构进行了研究。结果表明:施肥处理能够不同程度的增加土壤微生物的PLFA总量、细菌PLFA量和真菌PLFA量,其中化肥+有机肥处理下的土壤微生物PLFA总量,细菌PLFA量和真菌PLFA量增加作用比较明显,差异显著于对照处理;对不同施肥处理的复垦土壤微生物群落PLFA进行主成分分析也可得出化肥+有机肥处理下的土壤微生物的群落结构变化比较大,从因子载荷图上进一步分析可知,化肥+有机肥处理下的土壤中代表真菌的不饱和脂肪酸C18∶2ω6t、C18∶3ω6、18∶1ω9t、18∶3ω3的含量较高,这些脂肪酸将化肥+有机肥处理与单施化肥和对照处理区分开来,产生了明显的优势种群。土壤PLFA总量与土壤有机质、碱解氮、速效磷和速效钾含量都有很好的相关性,相关系数分别为0.76,0.85,0.67和0.67。
Phospholipid fatty acid (PLFA) analysis method was used to study the microbial community structure of reclaimed soil in the coal mining subsidence area of the Changzhi Wuyuan coal mine in Shanxi Province. The results showed that fertilization could increase PLFA amount, bacterial PLFA amount and fungal PLFA amount of soil microorganisms to some extent. Among them, PLFA amount, bacterial PLFA amount and fungal PLFA amount increased significantly under chemical fertilizer + organic manure treatment , And the difference was significant in comparison with the control. The principal component analysis of PLFA in different fertilization treatments also showed that the community structure of soil microorganisms under the treatment of chemical fertilizers and organic manures was relatively large. From the further analysis of the factor load map, we can see , And the content of unsaturated fatty acids C18: 2ω6t, C18: 3ω6, 18:1ω9t, 18:3ω3 which represent fungi in the soil treated by chemical fertilizer + organic manure is relatively high. These fatty acids combined the treatment of chemical fertilizer + organic fertilizer with that of chemical fertilizer and control Distinction between treatments resulted in a distinct predominant population. The total amount of soil PLFA had good correlation with soil organic matter, available nitrogen, available phosphorus and available potassium, with correlation coefficients of 0.76, 0.85, 0.67 and 0.67 respectively.