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目的:观察诱导分化剂苯乙酸(Phenylacetate,PA)抑制结直肠癌细胞HCT-8增殖过程中C-myc基因表达的变化。方法:应用噻唑蓝(MTT)比色法,以1.0、2.0、3.0、4.0、5.0mmol/L的PA作用于HCT-8细胞,分别于24、48、72h对细胞增殖进行检测。应用原位分子杂交方法观察应用PA后对HCT-8细胞C-myc mRNA表达的情况。结果:1.0～5.0mmol/LPA作用HCT-8细胞24～72h,随着PA浓度的增加或作用时间的延长,细胞生长抑制率明显增加,PA作用24h为5.1%～24.3%,48h为16.7%～72.3%,72h为30.2%～93.4%。PA治疗后HCT-8细胞C-myc mRNA阳性表达率为(12.05±7.92)%,显著低于非治疗组中的阳性率(55.15±21.64)%(P<0.01)。结论:PA可有效抑制结直肠癌HCT-8细胞的增殖,PA对C-myc基因具有明显的抑制作用。
OBJECTIVE: To observe the change of C-myc gene expression induced by phenylacetate (PA), an inhibitor of proliferation, in human colorectal cancer cell line HCT-8. Methods: HCT-8 cells were treated with 1.0, 2.0, 3.0, 4.0 and 5.0 mmol / L PA by MTT method. The cell proliferation was detected at 24, 48 and 72 hours respectively. In situ hybridization was used to observe the expression of C-myc mRNA in HCT-8 cells after PA administration. Results: After treated with 1.0-5.0 mmol / L LPA for 24-72 h, the growth inhibition rate of HCT-8 cells increased significantly from 5.1% to 24.3% at 24 h and 16.7% at 48 h with the increase of PA concentration or the prolongation of action time. ~ 72.3%, 72h 30.2% ~ 93.4%. The positive expression rate of C-myc mRNA in HCT-8 cells was (12.05 ± 7.92)% after PA treatment, which was significantly lower than that in non-treatment group (55.15 ± 21.64)% (P <0.01). Conclusion: PA can effectively inhibit the proliferation of colorectal cancer HCT-8 cells, PA has a significant inhibitory effect on C-myc gene.