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目的 :探讨红芪多糖 (HPS)对糖尿病 (DM)大鼠肾脏保护作用的机制。方法 :实验采用随机方法。左下腹腔注射链脲菌素 (STZ) 6 5 mg/ kg制备 DM大鼠模型 ,以 72 h后空腹血糖 >16 .7mm ol/ L 为模型成功标志。将 DM大鼠随机分为模型组、依那普利组及 HPS大、小剂量组。依那普利灌胃剂量为 7mg/ kg,HPS灌胃剂量为 15 0和 5 0 mg/ kg,余灌胃等量生理盐水 ,均每日 1次 ,连续 8周。灌胃 2周和 8周末次给药后禁食 12 h(不禁水 ) ,测血糖 ;分两批处死动物 ,取肾进行组织匀浆 ,测定一氧化氮 (NO)、一氧化氮合酶 (NOS)及超氧化物歧化酶 (SOD)、丙二醛 (MDA)等指标。结果 :HPS治疗后 DM大鼠血糖显著降低 ,且随治疗时间的延长 ,降糖作用逐渐明显。DM大鼠肾组织 NO和 NOS活性呈明显的早期升高、晚期下降趋势 ;使用 HPS2周时显著抑制了NO和 NOS活性的过分升高 ,8周时显著抑制了 NO和 NOS活性的持续降低。随病程延长 DM大鼠肾组织SOD活性呈逐渐下降、MDA呈逐渐升高的趋势 ;HPS治疗后肾组织 SOD活性明显增强 ,8周时有显著下降 ;MDA则呈下降趋势。以上作用大剂量 HPS组均优于小剂量组 ,呈明显的量效关系 ;依那普利组作用不明显。结论 :HPS能有效降低 DM大鼠血糖 ,改善和调节肾组织过氧化反应指标 ,可能对 DM肾脏损害有保护?
Objective: To investigate the mechanism of protective effect of polysaccharides from red buckwheat (HPS) on diabetic rats (DM). Methods : The experiment was conducted using a random method. The model of DM rats was prepared by intraperitoneal injection of streptozotocin (STZ) 65 mg/kg, and the fasting blood glucose was greater than 16.7 mm ol/L after 72 hours. DM rats were randomly divided into model group, enalapril group, and HPS large and small dose groups. The enalapril gavage dose was 7 mg/kg, the HPS gavage doses were 150 and 50 mg/kg, and the same amount of saline was administered by gavage once a day for 8 weeks. After 2 weeks and 8 weeks of oral ingestion, they were fasted for 12 hours (with water) and blood glucose was measured; the animals were sacrificed in two batches, and the kidneys were homogenized to measure nitric oxide (NO) and nitric oxide synthase ( NOS) and superoxide dismutase (SOD), malondialdehyde (MDA) and other indicators. Results : The blood glucose of DM rats was significantly reduced after HPS treatment. With the prolongation of treatment time, the hypoglycemic effect was gradually obvious. The activity of NO and NOS in the kidneys of DM rats showed a significant early increase and late decline. The use of HPS 2 weeks significantly inhibited the over-elevation of NO and NOS activity. At 8 weeks, the activity of NO and NOS decreased significantly. With the prolongation of the course of disease, the activity of SOD in kidneys of DM rats gradually decreased and the MDA increased gradually. After SOS treatment, the activity of SOD in kidneys significantly increased, and decreased significantly at 8 weeks. MDA showed a decreasing trend. The above effect of high-dose HPS group was superior to the small-dose group, showed a significant dose-effect relationship; enalapril group had no significant effect. Conclusion : HPS can effectively reduce blood glucose in DM rats, improve and regulate the index of renal peroxidation, and may protect DM kidney damage.