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目的：明确寻常型银屑病( psoriasis vulgaris，PV)皮损组织与正常人皮肤中的差异表达蛋白。方法：收集PV皮损组织及正常人皮肤组织，提取蛋白，进行二维凝胶电泳，选择在PV皮损组织中明显差异表达的蛋白点，进行质谱和生物信息学分析。结果： PV患者角质层和真皮层二维凝胶电泳鉴别蛋白总数分别为(1961±21)和(1928±49)个，确定差异表达蛋白30个，其中14个被鉴定分型：包括角质1型细胞骨架( K1C10、K1C14、K1C15、K1C16)、角质2型细胞骨架( K2C1)，肌动蛋白( ARP 3，ACTA，ACTBM)，热休克蛋白( PHB，HSPβ1，CH60)，中心体蛋白( CP135)，膜联蛋白( ANXA4、ANXA5)。结论：差异表达蛋白可能在PV的病理形成中起重要作用，是潜在的PV诊断标记物。“,”Objective:To identify the differentially expressed proteins in psoriasis vulgaris (PV) lesions and normal human skin tissue to propose potential pathological markers in psoriasis vulgaris. Methods:PV le-sions and normal human skin tissues were collected and the proteins were extracted. And then, the proteins were resolved by immobilized pH gradient-based two-dimensional electrophoresis ( 2-DE) . The differentially expressed proteins were analyzed by matrix-assisted laser desorption /ionization time of flight mass spectrome-try (MALDI-TOF-TOF-MS) and bioinformation. Results: Well-resolved 2-DE profiles of the epidermal and dermal layer were obtained,and average protein dots were (1969±21) and (1928±49) respectively;30 differentially expressed proteins were identified,14 of which were well characterized,including keratin type I,actin,heat shock protein,centrosomal protein and annexin. Conclusion:The differentially expressed proteins may play an important role in the pathogenesis of psoriasis and may be potential biomarkers in the diagnosis of PV.